Journal: Molecular Therapy. Methods & Clinical Development
Article Title: Monolayer platform using human biopsy-derived duodenal organoids for pharmaceutical research
doi: 10.1016/j.omtm.2021.05.005
Figure Lengend Snippet: Comprehensive gene expression analysis Human adult duodenal biopsies (five-donor pool, adult duodenum), the human duodenal organoid (organoid), the human duodenal organoid-derived monolayer at 8 days after seeding (organoid-monolayer), and Caco-2 cells (Caco-2) were subjected to DNA microarray analysis. (A) Heatmap and hierarchical clustering of gene expression levels obtained by microarray analysis. (B) Violin plots to visualize the expression levels of genes in each gene cluster. Statistical significance was evaluated by one-way ANOVA followed by a Tukey’s post hoc test. Groups that do not share the same letter had significantly different results (p < 0.05).
Article Snippet: Then, cRNA amplifying, labeling, hybridizing, and analyzing were performed at Takara Bio (Shiga, Japan) using a SurePrint G3 Human Gene Expression 8×60K v3 microarray (G4851C; Agilent Technologies, CA, USA).
Techniques: Expressing, Derivative Assay, Microarray